1036-pET30-RecA
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Cloning of 1036-pET30-RecA
[edit]Purpose: Cloning of 1036-pET30-RecA
Date: 2012-4-2---2012-4-6
Notebook:0
Page:
Clone ID:1036
Experimenters: yingying
Results
[edit]cloning
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PCR using Top10 genomic DNA
RecA-Nde1-F
actagcgCATATGGCTATCGACGAAAACAAAC
RecA-Xho1-R
(GCAGAAACTAACGAAGATTTTCTCGAG cgaggc)
gcctcgCTCGAGAAAATCTTCGTTAGTTTCTGC
digest:
| band 3 |
band 4 | |
| PCR product |
pET30 | |
| enzymes |
Nde1 + Xho1 | |
Colony Screening
[edit]Date: 2012-4-6
Make miniprep and digest with Nde1 + Xho1
clone 1,2,3,4,5,6 may be right, need to sequence
Result intepretation/Summary
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Conclusion
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