1037 pET30b-T7ligase

Cloning of 1037 pET30b-T7ligase

Purpose: Cloning of 1037 T7 ligase by gene synthesis

Date: 2012-3-25-

Notebook: 

Page:

Clone ID:1037

Experimenters: Paul

Results

Gene synthesis

Date:2012-3-25

1: first fragment, oligo 1-14

2: 2nd fragment, oligo 15-30

Lane 6 & 7: 2nd round PCR using combined fragments (1 &2)

1: Nde1+ Xho1 digestion of gene synthesis fragment

2: pET30 digestion with Nde1 + Xho1.