1048-pET30b-RNaseI
Cloning of 1048-pET30b-RNaseI
[edit]Purpose: Cloning of1048-pET30b-RNaseI
Date: 2012-3-27--2012-3-30
Notebook:
Page:003783
Clone ID:1048
Experimenters: yingying
Results
[edit]cloning
[edit]Date:2012-3-27
Fragment1: RnaseI-1 ~RnaseI-20, ~650 bp,gene synthesis
Fragment 2: RnaseI-21~40, ~740 bp,gene synthesis
PCR 2 fragments and then using Gibson mix to join them
digest :
| band 3 |
band 4 | |
| Gibson product |
pET30b | |
| enzymes |
| |
Colony Screening
[edit]Date: 2012-3-26
PCR screen with T7 F+RnaseI-40
no product
Result intepretation/Summary
[edit]Conclusion
[edit]<br>
<br>
<br>
Cloning of 1048-pET30b-RNaseI
[edit]Purpose: Cloning of1048-pET30b-RNaseI
Date: 2012-3-30--2012-4-2
Notebook:
Page:003789
Clone ID:1048
Experimenters: yingying
Results
[edit]cloning
[edit]Date:2012-3-30
Fragment1: RnaseI-1 ~RnaseI-20, ~650 bp,gene synthesis
Fragment 2: RnaseI-21~40, ~740 bp,gene synthesis
PCR 2 fragments then
Fragment1+Fragment 2 as template
RnaseI-1+RnaseI-40 as primers
| band 3 |
band 4 | |
| |
||
| enzymes |
| |
Colony Screening
[edit]Date: 2012-4-3
miniprep and digest with Nde1+ Xho1
no band
Result intepretation/Summary
[edit]Conclusion
[edit]<br>
<br>
<br>
Cloning of 1048-pET30b-RNaseI
[edit]Purpose: Cloning of1048-pET30b-RNaseI
Date: 2012-4-4--2012-4-10
Notebook:
Page:
Clone ID:1048
Experimenters: yingying
Results
[edit]cloning
[edit]Date:2012-3-27
Fragment1: RnaseI-1 ~RnaseI-20, ~650 bp,gene synthesis
Fragment 2: RnaseI-21~40, ~740 bp,gene synthesis
PCR 2 fragments then
Fragment1+Fragment 2 as template
RnaseI-1+RnaseI-40 as primers
digest :
| band 5 |
band 6 | |
| PCR product |
pET30b | |
| enzymes |
Nco1+ Xho1 | |
Colony Screening
[edit]Date: 2012-4-10
clone1,2,3,5,6,8 may be right, need to sequence
Result intepretation/Summary
[edit]Conclusion
[edit]<br>
<br>
<br>