1096-pET28b-T3-Ligase
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Cloning of 1096-pET28b-T3-Ligase
[edit]Purpose: Cloning of 1096-pET28b-T3-Ligase
Date: 2012-11-19--2012-11-27
Notebook:0
Page:
Clone ID:1096
Experimenters: yingying
Results
[edit]cloning
[edit]Gene synthesis of Fragment1 and 2.
Join them by 2nd round PCR
| lane |
3 |
4 |
| PCR product |
1027,pET28b-LambdaBet | |
| enzymes |
Nde1+Xho1 | |
Colony Screening
[edit]Date: 2012-11-26
mini prep and digest with Nde1+Xho1
T3 1-1 +T7 Term
clone 2,3,4,6,7,8 right ,need to sequence
2012-11-28
mini prep and digest with Nde1+Xho1
Result intepretation/Summary
[edit][edit]
- <br>
Conclusion
[edit]<br>