10 X high pH PCR buffer

10X Buffer:

300mM Tris pH 10.0 or pH 11.8
100mM KCl;
100mM Ammonium Sulfate
20mM Magnesium Sulfate
1% Trition X-100
1mg / ml nuclease-free bovine serum albumin (BSA).
High pH PCR Reaction Buffer for High Performance PCR