1102-pLVX-CMV5-MCS-IRES-mCherry

Cloning of pLVX-CMV5-MCS-IRES-mCherry(1102)
Purpose: to clone pLVX-CMV5-MCS-IRES-mCherry
Date: 2012-4-18 to 2012-4-23
Notebook: Xiaojing Wang
Page: 004227
Clone ID: 1102
Experimenters: Xiaojing Wang
Results

digest pLex-CMV5-CuO, 448 with Nde1 + EcoR1--> 1kb CMV5 promoter

digest pLVX-CAG-MCS-IRES-mCherry, 1060 with Nde1 + EcoR1-->vector

ligate and transform STBL3

Select 6 clones to mini-prep
1102 plasmids are digested by Nde1 + EcoR1

Clone 1102-2,-3,-4,-5 may be correct.