1103-pLVX-CMV5-MCS2-IRES-mCherry

Cloning of pLVX-CMV5-MCS2-IRES-mCherry(1103)
Purpose: to clone pLVX-CMV5-MCS2-IRES-mCherry
Date: 2012-4-26 to 2012-4-30
Notebook: Xiaojing Wang
Page: 004232
Clone ID: 1103
Experimenters: Xiaojing Wang
Results

digest vector (pLVX-CMV5-MCS-IRES-mCherry, 1102) with BamH1 + Spe1,

ligate to annealed oligos:

pLVX-CMV5-MCS2-T

pLVX-CMV5-MCS2-B ,transform STBL3

Select 6 clones to mini-prep
1103 plasmids are digested by Nde1 + Pac1

1103-1~-6 may be correct.