1105 pFastBacDual-TagGFP

Cloning of pFastBacDual-TagGFP(1105)
Purpose: to clone pFastBacDual-TagGFP
Date: 2012-7-12 to 2012-7-16
Notebook: Xiaojing Wang
Page: 005294

Clone ID: 1105
Experimenters: Xiaojing Wang

Results

PCR TagGFP from 109, pET28b-TagGFP
8685-TagGFP-F Xho1 HindIII 22nt IRES Kozak
TagGFP atcg CTCGAG AAGCTT TTTATAATTTCTTCTTCCAGAA GCCACC ATG AGC GGG GGC GAG
TagGFP-KpnI-R
(CATGGACGAGCTGTACAGGTAA GGTACC atcgac)
gtcgatGGTACCTTACCTGTACAGCTCGTCCATG

digest with Xho1 + Kpn1, clone into 403 pFastBac-Dual

ligate and transform DH5alpha

Pick up 6 clones to mini-prep
1105 plasmids are digested by Xho1 + Kpn1

Clone 1105-1,-2,-3,-4,-5,-6 may be correct.

Pick up 1105-1 to sequence.