1127 pEC-T4RNA-Lig2K227Q
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Cloning of 1127 pEC-T4RNA-Lig2K227Q
[edit]Purpose: Cloning of 1127 pEC-T4RNA-Lig2K227Q
Date: 2, 2013
Notebook:
Page:
Clone ID: 1127
Experimenters: shanyi
Results
[edit]Cloning
[edit]PCR
1: T4RNA-lig-Nde1-F + T4RNAlig-K227Q-R1 using 1123 as template, not more than 20 cycles
2: T4RNA-lig-Nde1-F + T4RNAlig-K227Q-R2 using PCR product from PCR reaction 1 as template
Clone into pEC Nde1 + Xho1
ligate and transform,
Colony Screening
[edit]Nde1 + Xho1
Result intepretation/Summary
[edit]
Conclusion
[edit]