1133 pET28b-M1wt

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5/5/2012

PCR amplification, 25 ul, using Kapa HiFi

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digest gel-purified PCR product and pET28b with Nde1 + Xho1

ligate and transform DH5alpha

Select 6 clones to mini-prep
1133 plasmids are digested by Nde1+Xho1


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--Excellgen (talk) 11:12, 5 February 2016 (EST)

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