1140 pTXB1-polyAPol
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Cloning of 1140 pTXB1-polyAPol
[edit]Purpose: Cloning of 1140 pTXB1-polyAPol
Date: July 23, 2012
Notebook:
Page:
Clone ID: 1140
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: July 23, 2012
Expected size 1398 bp, see lanes 5 and 6
PCR from MG1665 genomic DNA
polyAPol-Nde1-F
cgagca CATATGatttttacccgagtcgctaatttttg
polyAPol-Xho1-R
(caccacgtcgtgagggtaccgca CTCGAG atacta)
tagtatCTCGAGtgcggtaccctcacgacgtggtg
Clone into Nde1 + Xho1 1254 pTXB1-PT-ELK
See lane 9 (insert and vector digested together - top is vector bottom is insert)
Cut out both bands and ligate together to transform
Colony Screening
[edit]Date: July 27, 2012
Digested MiniPrep with RE Nde1 and Xho1
Expected size 1398 bp, see lanes 1-6
Samples 3, 5 and 6 may be right, submit sample 5 to sequencing
Result intepretation/Summary
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Conclusion
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