1146 1494 1672 1764 1891 1915 1931 1932 1944 1946 1947
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Protein Expression
[edit]2014/5/30
SHANYI
| Lane |
Name |
Protein Name |
Clone ID |
Host |
Induction |
SAMPLE |
pI/Mw |
express |
| 1 |
pT7A3E-HC-Alk-PPtase |
Alk-PPtase |
1146 |
15 ml, Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
5.18/48 |
yes |
| 2 |
pT7A3-UDG |
UDG |
1494 |
15 ml,
Nico21(DE3)prareplysS |
0.3mM IPTG RT O/N |
s |
6.18/28.8 |
no, no expression in Bl21(DE3)pareplysS too |
| 3 |
pT7A3-Afu-UDG |
Afu-UDG |
1672 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
S |
7.66/24.9 |
YES |
| 4 |
pT7A3-T4-Pnk |
T4-Pnk |
1764 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
6.26/28.1 |
yes |
| 5 |
pET28b-TAB5 |
TAB5 |
1891 |
15 ml,
Tuner(DE3)plysN |
0.3mM IPTG RT O/N |
s |
5.7/40.3 |
no |
| 6 |
pT7A3-8H-HC-VV-PolyA-Pol_S |
VV-PolyA-Pol_S |
1915 |
15 ml,
Tuner(DE3)plysN |
0.3mM IPTG RT O/N |
s |
9.52/40.3 |
yes |
| 7 |
pT7A3-T4-RNA-Lig2 |
T4-RNA-Lig2 |
1931 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
6.28/39.1 |
no, has expression with MBP(1123 pT7A3-MBP-(3C)-T4-RNA-Lig2) |
| 8 |
pT7A3E-T4_RNA_Ligase_ 2_Truncated |
T4_RNA_Ligase_ 2_Truncated |
1932 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
6.16/29.1 |
yes |
| 9 |
pageRuler Plus Prestained protein ladder |
|
|
|
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| 10 |
pT7A3E-polyAPol |
polyAPol |
1944 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
S |
9.6/55.7 |
no, 1513-pT7A3-polyAPol cannot express too |
| 11 |
pT7A3E-bTdT |
bTdT |
1946 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
S |
7.67/45.7 |
not sure |
| 12 |
pT7A3E-Exo-1 |
E coli exonuclease I |
1947 |
15 ml,
Nico21(DE3)plysN |
s |
5.71/56.2 |
yes |