1149 1149p 1274 1291 1292 1322 1571 1574 1588 1869 1910

Protein Expression

2014/7/24
SHANYI

Lane	Name	Protein Name	Clone ID	Host	Induction	Purification	pI/Mw	express	sample
1	pT7A3E-6H-HC-Xis		1149	5 ml, Nico21(DE3)plysN	0.2 mM IPTG  RT O/N	Ni beads	10.93/10
2	pT7A3E-6H-HC-Xis		1149	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT O/N	Ni beads	10.93/10	yes	pellet
3	pT7A3-RnaseH2		1274	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT O/N	Ni beads	11/13.3	yes
4	PageRuler Plus Prestained protein ladder
5	pET30b-TAT-NLS-sso7d		1291	5 ml, Nico21(DE3)plysN	0.4 mM IPTG RT O/N	Ni beads	10.7/13.3	yes
6	pET30b-PTD-DRBD-sso7d		1292	5 ml, Nico21(DE3)plysN	0.4mM IPTG RT O/N	Ni beads	10.7/24.6
7	pT7A3-mutY		1322	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	Ni beads	8.76/41.2
8	pT7A3-APE1		1571	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	Ni beads	8.08/37.7	yes
9	pT7A3E-Nu1		1574	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	Ni beads	5.51/22.1
10	pET30bHC-Exo-VII		1588	5 ml, Nico21(DE3)plysN	0.4mM IPTG RT O/N	Ni beads	9.94/52.9 cell lysed
11	pT7A30-p22Lyso		1869	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	Ni beads	9.33/17.2	yes
12	pT7A3E-BsuPo		1910	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	Ni beads	5.24/70	yes