1200 pDB.His.MBP-M1wt
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Cloning of pDB.His.MBP-M1wt(1200)
Purpose: to clone pDB.His.MBP-M1wt
Date: 2012-5-22 to 2012-5-24
Notebook: Xiaojing Wang
Page: 004250
Clone ID: 1200
Experimenters: Xiaojing Wang
Results
digest pDB.His.MBP-M1wt 1133 with Nde1 + Xho1--> inseert
digest 360 pDB.His.MBP with Nde1 + Xho1--> vector
Ligate and transform
Select 5 clones to mini-prep
1200 plasmids are digested by Nde1 + Xho1
1200-1~-6 may be all correct.