1201 pCMV5-CymR
Jump to navigation
Jump to search
Cloning of pCMV5-CymR(1201)
Purpose: to clone pCMV5-CymR
Date: 2012-6-4 to 2012-6-
Notebook: Xiaojing Wang
Page: 004257
Clone ID: 1201
Experimenters: Xiaojing Wang
Results
digest 1202 pDNA3.1-CymR with Nde1 + BamH1-->Vector
digest 448 pLex-CMV5-CuO with Nde1 + BamH1--> insert (promoter)
Ligate and transform
Select 6 clones to mini-prep
1201 plasmids are digested by Nde1 + BamH1
1201-1,-2 may be correct.