1202 pDNA3.1-CymR
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Cloning of pDNA3.1-CymR(1202)
Purpose: to clone pDNA3.1-CymR
Date: 2012-5-23 to 2012-5-
Notebook: Xiaojing Wang
Page: 004252
Clone ID: 1202
Experimenters: Xiaojing Wang
Results
digest pCDNA3.1, 335 with BamH1 + Xho1--> vector
digest 457 pET28b-CymR or pLenti-CymR 458 with BamH1 + Xho1-->insert
Ligate and transform
Select 6 clones to mini-prep
1202 plasmids are digested by BamH1 + Xho1
1202-6,-9,10 may be correct.
1202-6 is digested by Nde1+BamH1 --->band1
1202-6 is digested by Nde1+Xho1 --->band2
