1203 pET42b-scIHF2

Cloning of pET42b-scIHF2(1203)
Purpose: to clone pET42b-scIHF2
Date: 2012-5-23 to 2012-5-25
Notebook: Xiaojing Wang
Page: 004252
Clone ID: 1203
Experimenters: Xiaojing Wang
Results

Digest pET42b with Nco1 + Xho1--> vector
digest 1078 pET28b-scIHF2 with Nco1 + Xho1--> insert 627bp

Ligate and transform
Select 6 clones to mini-prep
1203 plasmids are digested by Nco1 + Xho1

1203-1,-2,-3,-5 may be correct.

Protein Expression

induction, 37 oC 5 hours

Lane	ID	Name	Pi, MW	Induction	Sample	Expression
1	1708	pET28b-scIHF2			S
2	1203	pET42b-scIHF2			S
3	1074	pET28b-IHFab			S
4	1186	pDB.His.MBP-Xis			S
5	406	pET28b-Int			S
6	1185	pDB.His.MBP-Int
7	1708	pET28b-scIHF2	9.60 / 22.8		P	Y
8	1203	pET42b-scIHF2	8.38 / 54.2		P	Y
9	1074	pET28b-IHFab	9.20 / 25.3		P	N
10	1186	pDB.His.MBP-Xis	6.62 / 52.1		P	Y
11	406	pET28b-Int	9.53 / 42.5		P	N
12	1185	pDB.His.MBP-Int	7.33 / 83.7		P	Y
M		PageRuler Plus	250, 130, 00, 70, 55, 35, 25, 15, 10