1220 pFUSE2-CLIg-hk-zeo

Cloning of 1220 pFUSE2-CLIg-hk-zeo

Purpose: make pFUSE2-CLIg-hk-zeo vector

Date: 2012-6-3

Notebook: 

Page:

Clone ID:1220

Experimenters: Paul

Results

cloning

6/3/2012

digest 1161 pFUSE-CHIg-hG1 (Lane 4, 5, 6) with Hpa1 + Nde1 ---> zeocin insert
the size of zeocin insert should be ~1.6 kb. this one looks like 5.5 kb, maybe not digested?

digest pFUSE2-CLIg-hk 1162 with Hpa1 + Nde1 ---> vector

ligate, transform DH5alpha

Colony Screening

Date:

•