1231-pCMV5-MCS-IRES-SVLT

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Cloning of 1231-pCMV5-MCS-IRES-SVLT

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Purpose: Cloning of 1231-pCMV5-MCS-IRES-SVLT

Date: 2012-7-24----2012-7-27

Notebook:0

Page:

Clone ID:1231

Experimenters: yingying

Results

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subcloning

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  2
1228,pCMV5.3-IRES-SVLT vector
enzymes
        BamH1 + Not1

ligate with annealed oligos

1228-MCS-T C8 GATCC CTTAAG TTAATTAA GAGCTC GCTAGC ATCGAT GC
1228-MCS-B C9 GGCCGCATCGATGCTAGCGAGCTCTTAATTAACTTAAGG


Colony Screening

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Date: 2012-7-27

Make miniprep and digest with Nhe+EcoR1

no insert



Result intepretation/Summary

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  • <br>

Conclusion

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