1236 pCDNA3.1-AvPAL-2
Jump to navigation
Jump to search
pCDNA3.1-AvPAL-2
[edit]Date: 2012-6-10
Notebook:
Page:
Clone ID: 1236
Experimenters: Paul
Cloning
[edit]6/10/2012. Synthesize fragment 2 using KAPA, 20 cycles.
digest PCR product with BamH1+ EcoR1
digest pCDNA3.1 with BamH1+ EcoR1, ligate and transform Top10
Colony Screening by Restriction Digestion
[edit]
Digested samples with BamHI and EcoRI, positive in samples 1, 3, 4, 5 and 6.
Submitted sample 5 for sequencing.This clone is good. Make glycerol stock