1254-pTXB1-PT-ELK
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Cloning of 1254-pTXB1-PT-ELK
[edit]Purpose: Cloning of 1254-pTXB1-PT-ELK
Date: 2012-7-5---2012-7-9
Notebook:0
Page:
Clone ID:1254
Experimenters: yingying
Results
[edit]cloning
[edit]
| band 3 |
band 4 | |
| PCR product |
pTXB1 1126 | |
| enzymes |
Pst1 + BamH1 | |
Colony Screening
[edit]Date: 2012-7-9
Make miniprep and digest with Pst1 + BamH1
no insert
no insert
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]
Cloning of 1254-pTXB1-PT-ELK
[edit]Purpose: Cloning of 1254-pTXB1-PT-ELK
Date: 2012-7-10---2012-7-12
Notebook:0
Page:
Clone ID:1254
Experimenters: yingying
Results
[edit]cloning
[edit]
| lane 1 |
lane 2 | |
| PCR product |
pTXB1 1126 | |
| enzymes |
Pst1 + BamH1 | |
Colony Screening
[edit]Date: 2012-7-12
Make miniprep and digest with Pst1 + BamH1
clone 2,3,6,8 may be right
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]
Cloning of 1254-pTXB1-PT-ELK
[edit]Purpose: Cloning of 1254-pTXB1-PT-ELK
Date: 2012-7-17---2012-7-19
Notebook:0
Page:
Clone ID:1254
Experimenters: yingying
Results
[edit]subcloning
[edit]
| band 1 |
band 2 | |
| 1254-2 |
1255 pTXB1-C6H2 | |
| enzymes |
Pst1 + BamH1 | |
Colony Screening
[edit]Date: 2012-7-19
Make miniprep and digest with Pst1 + BamH1
clone 3 ,4,5,7,8 may be right
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]