1270 pTXB1-RNaseT1
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Cloning of 1270 pTXB1-RNaseT1
[edit]Purpose: Cloning of 1270 pTXB1-RNaseT1 by gene synthesis
Date: July 26, 2012
Notebook: 0
Page:
Clone ID: 1270
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: July 26, 2012
Amplified DNA sequence by gene synthesis, expected size ~400 bp (see lane 2)
Cut out band and purified
Digested with vector 1254 with RE Xho1 and Nde1
See lanes 3 (insert) and 4 (vector) Cut out, purify and ligate together.
Transform
Colony Screening
[edit]Date: July 30, 2012
Digested MiniPrep with Nde1 and Xho1 RE
Expected size 400 bp
Samples 4 (lane 8), 4 (lane 10) and 6 (lane 12) may have positive inserts
Result intepretation/Summary
[edit]