1274-pT7A3-RnaseH2
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Cloning of 1274-pT7A3-RnaseH2
[edit]Purpose: Cloning of 1274-pT7A3-RnaseH2
Date: 2014-7-01---2014-7-2
Notebook:0
Page:
Clone ID:1274
Experimenters: LIANGHUO
Results
[edit]cloning
[edit]Cloning Method
PCR using genomic DNA from Escherichia coli str. K-12 substr. MG1655
RnaseH2-Nde1-F AGCTCTCATatgatcgaatttgtttatccgcac
RnaseH2-Xho1-R gtctcgctcgagggacgcaagtcccagtgcgcgt
clone into Nde1 + Xho1 digested 1892-pT7A3E
1274 DIGESTED
[edit]
Colony Screening
[edit]Date: 2014-7-01
Make miniprep and digest with Nde1 + XhoI
SELECT CLONE4 FOR SEQUENCING
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Result intepretation/Summary
[edit]Conclusion
[edit]