1282-pMAL-p5E-AvPAL
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Cloning of 1282-pMAL-p5E-AvPAL
[edit]Purpose: Cloning of 1282-pMAL-p5E-AvPAL
Date: 2012-7-30---2012-7-30
Notebook:0
Page:
Clone ID:1282
Experimenters: yingying
Results
[edit]subcloning
[edit]
| lane |
1 |
2 |
| 1023 pET8XHis-AvPAL |
1281 pMAL-p5E | |
| enzymes |
Nco1 + Xho1 |
Nco1 + Sal1 |
Colony Screening
[edit]Date: 2012
Make miniprep and digest with
wrong antibiotic plate, repeat again
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>
<br>
<br>
<br>
Cloning of 1282-pMAL-p5E-AvPAL
[edit]Purpose: Cloning of 1282-pMAL-p5E-AvPAL
Date: 2012-8-6---2012-8-8
Notebook:0
Page:
Clone ID:1282
Experimenters: yingying
Results
[edit]subcloning
[edit]
| band 1 |
band 2 | |
| 1023 pET8XHis-AvPAL |
1281 pMAL-p5E | |
| enzymes |
Nco1 + Xho1 |
Nco1 + Sal1 |
Colony Screening
[edit]Date: 2012-8-8
Make miniprep and digest with Nco1+EcoR1
Clone 2 3 5 6 right
c
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>
<br>
<br>