1298 pET-6H-MPGa
Cloning of 1298 pET-6H-MPGa
[edit]Purpose: Cloning of 1298 pET-6H-MPGa
Date: August 13, 2012
Notebook:
Page:
Clone ID: 1298
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: August 13, 2012
Cloning Method
PCR gene synthesis, see lane 3, expected size 114 bp
Cut out and purify DNA
Digest with Nco1 + Nde1, ligate to Nco1 + Nde1 digested 1128 pDB.His.PfuMBP-(tev) overnight, transform
Date: August 14, 2012
Insert disappeared after digestion, need to reamplify, see lane 2
Date: August 15, 2012
Digest with Nco1 + Nde1 (lane 3), ligate to Nco1 + Nde1 digested pDB.His.MBP, V14 (lane 4), transform
Date: August 17, 2012
Digest with Nco1 + Nde1 (lane 2), ligate to Nco1 + Nde1 digested pDB.His.MBP, V14 , transform
Colony Screening
[edit]Date: August 20, 2012
No inserts
Result intepretation/Summary
[edit]
Conclusion
[edit]
Cloning of 1298 pET-6H-MPGa
[edit]Purpose: Cloning of 1298 pET-6H-MPGa
Date: August 30, 2012
Notebook:
Page:
Clone ID: 1298
Experimenters: yingying lu
Results
[edit]Cloning
[edit]Date: August 30, 2012
Cloning Method
PCR gene synthesis, see lane 3, expected size 114 bp
Cut out and purify DNA
Digest with Nco1 + Nde1, ligate to Nco1 + Nde1 digested V14 pDB.His-MBP overnight, transform
Colony Screening
[edit]Date: 2012-9-4
no insert
Result intepretation/Summary
[edit]
Conclusion
[edit]
Cloning of 1298 pET-6H-MPGa
[edit]Purpose: Cloning of 1298 pET-6H-MPGa
Date: 2012-10-19---2012-10-23
Notebook:
Page:
Clone ID: 1298
Experimenters: shanyi& yingying
Results
[edit]Cloning
[edit]
Colony Screening
Date: 2012-10-22
5ul sample
2012-10-23
10ul sample
Result intepretation/Summary
[edit]
Conclusion
[edit]