1302 pET-6H-Penetratin
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Cloning of 1302 pET-6H-Penetratin
[edit]Purpose: Cloning of 1302 pET-6H-Penetratin
Date: August 13, 2012
Notebook:
Page:
Clone ID: 1302
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: August 13, 2012
Anneal two oligos
His-Penetratin-T
cATGGCACATCATCATCACCATCATCGTGGCATTAAATGGTTTGGTAATCGTCGCATGAAATGGAAAAAAca
His-Penetratin-B
tatgTTTTTTCCATTTCATGCGACGATTACCAAACCATTTAATGCCACGATGATGGTGATGATGATGTGc
Ligate to Nco1 +Nde1 digested 1128 pDB.His.PfuMBP-(tev), see lane 4
Transform
Date: August 15, 2012
Anneal two oligos
His-Penetratin-T
cATGGCACATCATCATCACCATCATCGTGGCATTAAATGGTTTGGTAATCGTCGCATGAAATGGAAAAAAca
His-Penetratin-B
tatgTTTTTTCCATTTCATGCGACGATTACCAAACCATTTAATGCCACGATGATGGTGATGATGATGTGc
Ligate to Nco1 +Nde1 digested pDB.His.MBP, V14, see lane 4
Transform
Colony Screening
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Result intepretation/Summary
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Conclusion
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