1303 pTXB1-Cre
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Cloning of 1303 pTXB1-Cre
[edit]Purpose: Cloning of 1303 pTXB1-Cre
Date: August 24, 2012
Notebook:
Page:
Clone ID: 1303
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: August 24, 2012
See lane 5
PCR using 298 pTriEx-HTNC as template
Cre-Nde1-F
cagtca catatg tcc aat tta ctg acc gta cac
Cre-Xho1-R
(gaagatggcgattagctcgag ctgaca)
tgtcagctcgagctaatcgccatcttc
clone into Nde1 + Xho1 digested 1254 pTXB1-PT-ELK
Digested DNA (lane 5) and vector 1254 pTXB1-PT-ELK with RE Nde1 + Xho1
Cut out and purify, ligate together, transform
Colony Screening
[edit]2012-8-27
Nde1 + Xho1
Result intepretation/Summary
[edit]
Conclusion
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