1319A pEC2-T4-BGT
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Cloning of 1319A pEC2-T4-BGT
[edit]Purpose: Cloning of1319 pEC2-T4-BGT==
Date: 2014-9----
Notebook:0
Page:
Clone ID:1319
Experimenters: shanyi
Results
[edit]CLONING
[edit]pGene synthesis Fragment 1,2
join two fragments by PCR
digest with Nde1 + XhoI
Colony Screening
[edit]Date: 2014_8
Make miniprep, use Nde1 + Xho1 to find positive clone
fix 1319-3pT7A3E-T4-BGT
[edit]pcr using
T4-BGT-1-1+T4-BGT-fix-1+T4-BGT-1-2+T4-BGT-2-16
digest with Nde1 + XhoI
Colony Screening
[edit]Date: 2014_8
Make miniprep, use Nde1 + Xho1 to find positive clone
use T7 term primer+T4-BGT-2-16 to do PCR
