1331 pLVX-PR8-HAwt-TagGFP
Cloning of 1331 pLVX-PR8-HAwt-TagGFP
[edit]Purpose: Cloning of 1331 pLVX-PR8-HAwt-TagGFP
Date: August 21, 2012
Notebook:
Page:
Clone ID: 1331
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: August 21, 2012
See lane 3
PCR using these primers
PR8-HAwt-Sal1-F
agctacGTCGACgccaccatgaaggcaaacctactggtc
PR8-HAwt-BamH1-R
(ctttgcagtgcagaatatgcatcgaggatcctcgaca)
tgtcgaggatcctcgatgcatattctgcactgcaaag
template: 57 pHW2000-PR8 HAwt
Digest PCR product with Sal1 + BamH1
Digest vector with Xho1 + BamH1
Digested insert (lane 5) with RE Sal1 and BamH1, digested vector 110 with RE Xho1 and BamH1
LIgate together and transform
Colony Screening
[edit]Date: August 24, 2012
Digested MiniPrep with RE Xho1 and BamH1
Sent sample 1 to sequencing
Result intepretation/Summary
[edit]
Conclusion
[edit]
Cloning of 1331 pLVX-PR8-HAwt-TagGFP
[edit]Purpose: Cloning of 1331 pLVX-PR8-HAwt-TagGFP
Date: August 30, 2012
Notebook:
Page:
Clone ID: 1331
Experimenters: Yingying lu
Results
[edit]Cloning
[edit]Date: August 30, 2012
PCR using these primers
PR8-HAwt-Sal1-F
agctacGTCGACgccaccatgaaggcaaacctactggtc
PR8-HAwt-BamH1-R
(ctttgcagtgcagaatatgcatcgaggatcctcgaca)
tgtcgaggatcctcgatgcatattctgcactgcaaag
template: 57 pHW2000-PR8 HAwt
Digest PCR product with Sal1 + BamH1
Digest vector with Xho1 + BamH1
Colony Screening
[edit]Date: 2012-9-4
Digested MiniPrep with
Result intepretation/Summary
[edit]
Conclusion
[edit]