1332 pLVX-PR8-HA100S-TagGFP
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Cloning of 1332 pLVX-PR8-HA100S-TagGFP
[edit]Purpose: Cloning of 1332 pLVX-PR8-HA100S-TagGFP
Date: August 21, 2012
Notebook:
Page:
Clone ID: 1331
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: August 21, 2012
See lane 4
Cloning Method
PCR using these primers
PR8-HAwt-Sal1-F
agctacGTCGACgccaccatgaaggcaaacctactggtc
PR8-HAwt-BamH1-R
(ctttgcagtgcagaatatgcatcgaggatcctcgaca)
tgtcgaggatcctcgatgcatattctgcactgcaaag
template: 56 pHW2000-PR8 HA100S
Digest PCR product with Sal1 + BamH1
Digest vector with Xho1 + BamH1
Digested insert (lane 7) with RE Sal1 and BamH1, digested vector 110 with RE Xho1 and BamH1 (lane 8)
LIgate together and transform
Colony Screening
[edit]Date: August 24, 2012
Digested MiniPrep with RE Xho1 and BamH1
Sent sample 4 to sequencing
Result intepretation/Summary
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Conclusion
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