1333 pLVX-CA 04-HAwt-TagGFP
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Cloning of 1333 pLVX-CA 04-HAwt-TagGFP
[edit]Purpose: Cloning of 1333 pLVX-CA 04-HAwt-TagGFP
Date: August 21, 2012
Notebook:
Page:
Clone ID: 1333
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: August 21, 2012
See lane 5
PCR using these two oligos
TX_05-HAwt-Xho1-F
ctgaca CTC GAG gcc acc atgaaggcaatactagtagt tc
TX_05-HAwt-EcoR-R
(ctacagtgtagaatatgtatt CGA ATT CTG CAG T)
ACTGCAGAATTCGaatacatattctacactgtag
template : 55 pHW2000-CA/04 HAwt
Digested insert (lane 9) and vector 110 (lane 10) with RE Xho1 and EcoR1
Ligate together and transform
Colony Screening
[edit]Date: August 24, 2012
Digested MiniPrep with RE Xho1 and EcoR1
Sent sample 1 to sequencing
Result intepretation/Summary
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Conclusion
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