1350-pTT5-MCS
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Cloning of 1350-pTT5-MCS
[edit]Purpose: Cloning of 1350-pTT5-MCS
Date: 2012-8-30---2012-9-4
Notebook:0
Page:
Clone ID:1350
Experimenters: yingying
Results
[edit]cloning
[edit]PCR out CMV5 promoter tail using
Pcmv5-Xho1-F
GATCGGAAAACCTCTCGAGAAAG
CMV5-BglII-R
digest vector 390 SI1-Unc5b with Xho1 + BamH1. ---> vector
Colony Screening
[edit]Date: 2012-9-4
Make miniprep and digest with Xho1 + BamH1
Make miniprep and digest with Xho1 + Xba1
clone 1,3,4,5 may be right
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>