1351-pLVX-EF1-VP16-EBNA1c-Puro

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Cloning of 1351-pLVX-EF1-VP16-EBNA1c-Puro

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Purpose: Cloning of 1351-pLVX-EF1-VP16-EBNA1c-Puro

Date: 2012-10-2---2012-10-4

Notebook:0

Page:

Clone ID:1351

Experimenters: yingying

Results

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subcloning

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digest 219 pLentiGP (lane 3) with Xho1 + Mlu1--> GreenPuro with 22nt IRES insert
digest 1348 pLVX-EF1-VP16-EBNA1c (lane 2) with Xho1 + Mlu1--> vector


Colony Screening

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Date: 2012-10-4

Make miniprep and digest with Xho1 + Mlu1


Result intepretation/Summary

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  • <br>

Conclusion

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