1358 pLVX-PR8-NSwt-TagGFP
Cloning of 1358 pLVX-PR8-NSwt-TagGFP
[edit]Purpose: Cloning of 1358 pLVX-PR8-NSwt-TagRFP
Date: August 17, 2012
Notebook:
Page:
Clone ID: 1358
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: August 17, 2012
PR8-NSwt-Xho1-F
atcgacctcgaggccacc atg gac ccaaacactgtgtcaag
PR8-NSwt-BamH1-R
(cgggaacaattaggtcagaagtt gaG GAT CC tagcat)
atgctaGGATCCtcaacttctgacctaattgttcccg
Template 53 pHW2000-PR8 NSwt
Date: August 21, 2012
Digested insert (lane 13) and vector 110 (lane 14) with RE Xho1 and BamH1
Ligate together and transform
Colony Screening
[edit]Date: August 24, 2012
Digested MiniPrep with RE Xho1 + BamH1
Sent sample 1 to sequencing
Result intepretation/Summary
[edit]
Conclusion
[edit]
Cloning of 1358 pLVX-PR8-NSwt-TagGFP
[edit]Purpose: Cloning of 1358 pLVX-PR8-NSwt-TagRFP
Date: August 31, 2012
Notebook:
Page:
Clone ID: 1358
Experimenters: yingying lu
Results
[edit]Cloning
[edit]Date: August 31, 2012
Subclone from 1335 pLVX-PR8-NSwt-TagGFP using Xho1 + BamH1
Colony Screening
[edit]Date:2012-9-4
Digested MiniPrep with RE Xho1 + BamH1
clone 1,2,3,4,5 may be right
Result intepretation/Summary
[edit]
Conclusion
[edit]