1377-1378-1379-1380-1380-1381 Plasmid

1377-1378-1379-1380-1380-1381 Plasmid Maxiprep

Clones for maxiprep

9/24/2012

Select 1377-2, 1378-1, 1379-2, 1380-2, 1381-1 to make maxiprep using Qiagen maxiprep kit. 1381, 1392  using invitrogen maxiprep  kit

Clone ID	Parent	Clone Name	Expected Size (bp)	Actual Size on Gel (Kb)	Clone Identity	DNA conc	OD260/280	RNA
1377-1	1284		1647	2.5
1378-2	1276		1322	1.6
1379-2	1264		3205	4
1380-2	1269		2169	3
1381-1	1263		1586	2.5

1381 and 1392 using Invitrogen maxi prep kit have too much RNA. After treating  sample 1381 and 1392 with 0.5 ul RNAse (Qiagen) at 37oC , After 2-3 hours, there are still too much RNA on the gel, adding 10 ul (100mg/ml RNAse) into the sample and incubate at 37C for another 4-6 hours, precipitate DNA with isoprapanol. There isn't pure (Ratio is low), so the DNA reading is very low (minus value).There is too much RNase protein. Paul run the sample on the qiagen column again and there is no too much DNA left. Need re-do maxipre for this two sample.