1424-pCMV5-IgK-ATR-Fc-WPRE-oriP
Cloning of 1424-pCMV5-IgK-ATR-Fc-WPRE-oriP
[edit]Purpose: Cloning of 1424-pCMV5-IgK-ATR-Fc-WPRE-oriP
Date: 2012-10-24---2012-10-26
Notebook:0
Page:
Clone ID:1424
Experimenters: yingying
Results
[edit]cloning
[edit]
not the right size
Colony Screening
[edit]Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>
Cloning of 1424-pCMV5-IgK-ATR-Fc-WPRE-oriP =
[edit]Purpose: Cloning of 1424-pCMV5-IgK-ATR-Fc-WPRE-oriP
Date: 2012-11-15---2012-11-19
Notebook:0
Page:
Clone ID:1424
Experimenters: yingying
Results
[edit]cloning
[edit]PCR using the following oligos:
1 ul of 10 uM: ATR-IgG-k-BamH1-F1
1 ul of 1 uM: ATR-IgG-k-BamH1-F2
1 ul of 1 uM: ATR-IgG-k-BamH1-F3
1 ul of 1 uM: ATR-IgG-k-BamH1-F4
1 ul of 10 uM: ATR-Not1-R
template 1456,pLP-CMV-ATR
| lane |
1 |
2 |
| PCR product |
1387,pCMV5-hIgG-Hc-Dest-Fc(3c)-WPRE-OriP | |
| enzymes |
BamH1 + Not1 | |
Colony Screening
[edit]Date: 2012-11-19
Make miniprep and digest with BamH1 + Not1
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>
<br>
<br>
<br>
Cloning of 1424-pCMV5-IgK-ATR-Fc-WPRE-oriP
[edit]Purpose: Cloning of 1424-pCMV5-IgK-ATR-Fc-WPRE-oriP
Date: 2012-11-26
Notebook:0
Page:
Clone ID:1424
Experimenters: yingying
Results
[edit]cloning
[edit]PCR using the following oligos:
1 ul of 10 uM: ATR-IgG-k-BamH1-F1
1 ul of 1 uM: ATR-IgG-k-BamH1-F2
1 ul of 1 uM: ATR-IgG-k-BamH1-F3
1 ul of 1 uM: ATR-IgG-k-BamH1-F4
1 ul of 10 uM: ATR-Not1-R
template 1456,pLP-CMV-ATR
after digestion, the size became small
| lane 1 |
lane 2 | |
| PCR product |
1391 pCMV5-TPA-TagGFP-(3c)-Fc-WPRE-OriP | |
| enzymes |
BamH1 + Not1 | |
Colony Screening
[edit]Result intepretation/Summary
[edit]- &lt;br&gt;
Conclusion
[edit]&lt;br&gt;