142 pT7A3-MBP-GST-Avitag-BirA

3274 digest with Nde1-Nco1

1,2: 2ul digest in 50 ul vol

3, 2 ul digest in 20 ul vol

still ge multiple bands

precipitate 5 ul of plamid with NaoAc+ isppropanol, dissolve in H2O, digest again, still see mutiple bands