1441 pFus B9-SMN2-R2
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Cloning of 1441 pFus B9-SMN2-R2
[edit]Purpose: Cloning of 1441 pFus B9-SMN2-R2
Date: 2012-1---
Notebook:0
Page:
Clone ID:1441
Experimenters: shanyi
Results
[edit]Day2, pick white clones to PCR using primers pCR8_F1 and pCR8_R1 :
Day3
Miniprep the plasmids: Use enzymes AflII and XbaI to digest
Top1-5,Top1-8,Bottom1-1, Bottom2-1,Bottom2-4,Bottom2-5 may be right.
Sequence TOP1-5,BOTTOM1-1,BOTTOM2-1 with primers pCR8_F1 and pCR8_R1.
TOP1-5,BOTTOM1-1,BOTTOM2-1 are right.