1458 1779
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Protein Purification
[edit]2013/7/25
SHANYI
| Lane |
Name |
Protein Name |
Clone ID |
Host |
Purification |
pI/Mw |
Expression |
| 1 |
pT7A3-KOD |
KOD |
1458 |
Nico21DE3plysN |
10 ml Ni column Bio-Sil TSK 250 |
7.91 / 92215.77 |
yes |
| 2 | |||||||
| 3 | |||||||
| 4 | |||||||
| 5 | |||||||
| 6 | |||||||
| 7 | |||||||
| 8 |
PageRuler Plus Prestained protein ladder |
|
|
After TEVdigestion elute from Histrap |
|
||
| 9 |
pTCold-MBP-(3C-TEV)-NRF2 |
NRF2 |
1779 |
BL21DE3pRare2pLysS |
Flowthrough from Histrap and Amylose beads |
4.67 / 68345.09 |
|
| 10 |
Flowthrough from
Histrap |
4.67 / 68345.09 |
|||||
| 11 |
After TEVdigestion
elute from Histrap |
|
| ||||
| 12 |
After TEVdigestion
elute from Amylose beads |