1463-pAD5-PR8-HAin
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Cloning of 1463-pAD5-PR8-HAin
[edit]Purpose: Cloning of 1463-pAD5-PR8-HAin
Date: 2012-11-20--2012-11-26
Notebook:0
Page:
Clone ID:1463
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR using 1462 pHW2000-PR8/HAin as template and
PR8-HAwt-BamH1-F
PR8-HAwt-Not1-R
| lane | 5 | 6 |
| PCR product |
280 pAd5-CMV-MCS | |
| enzymes |
BamH1+Not1 | |
Colony Screening
[edit]Date: 2012-11-25
plasmid yield is very low
2012-11-26
clone1,2 3,4,5,6 need to sequece
Result intepretation/Summary
[edit][edit]
- <br>
Conclusion
[edit]<br>
<br>
<br>
<br>
Cloning of 1463-pAD5-PR8-HAin
[edit]Purpose: Cloning of 1463-pAD5-PR8-HAin
Date: 2012-11-29--2012-12-3
Notebook:0
Page:
Clone ID:1463
Experimenters: yingying
Results
[edit]cloning
[edit]PCR using 1462 pHW2000-PR8/HAin (new) as template and
PR8-HAwt-BamH1-F
PR8-HAwt-Not1-R
| lane | 5 | 6 |
| PCR product |
280 pAd5-CMV-MCS | |
| enzymes |
BamH1+Not1 | |
Colony Screening
[edit]Date: 2012-12-3
clone,2 ,4,5,6 need to sequece
Result intepretation/Summary
[edit][edit]
- &lt;br&gt;
Conclusion
[edit]&lt;br&gt;
&lt;br&gt;
<br>
<br>