1472-1476
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Cloning of 1472-1476
[edit]Purpose: Cloning of 1472-1476
Date: 2012-11-26---2012-11-29
Notebook:0
Page:
Clone ID:1472-1476
Experimenters: yingying
Results
[edit]subcloning
[edit]
| band 1 |
band 2 | |
| enzymes |
| |
Colony Screening
[edit]Date: 2012-11-18
Make miniprep and digest with Nhe1 + Kpn1
1472#3,4; 1473#1,2,3,4; 1475#1; 1476-#1; 1475#4 may be right, need to sequence
1472,1473,1474,1476 sequence result right, 1475 need to clone again
2012-12-4---2012-12-7
subclone : the same with above
mini prep and diges with Nhe1 + Kpn1
sequence result: 1475-6 right
Result intepretation/Summary
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Conclusion
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