1477 pEC-WPRE
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Cloning of 1477 pEC-WPRE
[edit]Purpose: Cloning of 1477 pEC-WPRE
Date: 2012-11-21-- Notebook:0
Page:
Clone ID:1477
Experimenters: SHANYI
Results
[edit]cloning
[edit]
Digest 1385 pCMV5-TPA-GNE-(3c)-Fc-WPRE-oriP with Eag1 + Xba1--insert digest vector 1454 pEC with Not1 + Xba1.
The band is not what we want.It should be 592bp.
Digest 1336pCMV5-Fc(3C)-Dest-WPRE with Not1 + Xba1--insert digest vector 1454 pEC with Not1 + Xba1---vector
Colony Screening
[edit]Date: 2012-12-
