1483-pT7A3-T4 Ligase

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Cloning of 1483-pT7A3-T4 Ligase

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Purpose: Cloning of 1483-pT7A3-T4 Ligase

Date: 2012-12-3---2012-12-5

Notebook:0

Page:

Clone ID:1483

Experimenters: yingying

Results

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subcloning

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lane 3
lane 4

240,pET28b-T4-Ligase
1279 pT7A3
enzymes
    NdeI + Xho1


Colony Screening

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Date: 2012-12-5

Make miniprep and digest withNdeI + Xho1

clone 1,3,4,5 right

Result intepretation/Summary

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Conclusion

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