1530 pET28b-Klenow3'5exo–

Cloning of pET28b-Klenow(3'->5'_exo–)

Purpose: Cloning of pET28b-Klenow(3'->5'_exo–)

Date: 1, 2013

Notebook: 

Page:

Clone ID: 1530

Experimenters: shanyi

Results

Cloning

PCR:two Fragments,then Join two fragment by PCR(25cycles)

Colony Screening

digest with Nde1 + Sal1, clone into pET28b

Cloning of pET28b-Klenow(3'->5'_exo–)

Purpose: Cloning of pET28b-Klenow(3'->5'_exo–)

Date: 1, 2013

Notebook: 

Page:

Clone ID: 1530

Experimenters: shanyi

Results

Cloning

PCR:use 1117 as template,two Fragments,then Join two fragment by PCR(25cycles)

cut out the first band(from up to down)

digest with Nde1 + Sal1,clone into pET28b

cut out the first band(from up to down)and the digested vector

Colony Screening

digest with Nde1 + Sal1,