1532 pT7A3-T4-DNA-helicase

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Cloning of 1532 pT7A3-T4-DNA-helicase

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Purpose: Cloning of 1532 pT7A3-T4-DNA-helicase

Date: Jan , 2013

Notebook: 

Page:

Clone ID: 1532

Experimenters: Shanyi

Results

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Cloning

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PCR


Nde1 + Xho1



Colony screen

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SEND 1532-4 TO SEQUENCE, IT IS T3 DNA ligase, SO CREATE A NEW NUMBER AS 1654.


PCR


digest with Nde1 + Xho1


Colony screen

[edit]

digest with Nde1 + Xho1

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