1544-pT7A3-VVCap-L
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Cloning of 1544-pT7A3-VVCap-L
[edit]Purpose: Cloning of 1544-pT7A3-VVCap-L
Date: 2013-1-10---2013-1-17
Notebook:0
Page:
Clone ID:1544
Experimenters: yingying
Results
[edit]cloning
[edit]
Nde1 + Xho1
Colony Screening
[edit]Date: 2012-1-17
Make miniprep and digest with Nde1 + Xho1
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]protein screen
[edit]2013/3/28
| lane |
name |
clone ID |
host |
induction |
purification |
MW |
| 1 |
pT7A3-sso7d-Native-ToughTaq |
1542 |
4 ml,
BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
62KD |
| 2 |
pT7A3-VVCap-L |
1544 |
4 ml,
BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
34KD |
| 3 |
pT7A3-VVCap |
1545 |
4 ml,
BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
34KD |
| 4 |
pT7A3-SOD |
1627 |
4 ml,
BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
16KD |
| 5 |
pCold-MBP-SM2 |
1679 |
4 ml,NiCO21(DE3)pLysN, |
0.2 mM IPTG 25 oC o/n |
Ni beads |
74KD |
| 6 |
pCold-GST-wtSM2 |
1680 |
4 ml,NiCO21(DE3)pLysN, |
0.2 mM IPTG 25 oC o/n |
get lysis supernatant to run gel |
94KD |