1547 PiggyBacHR-SMN1-3HA
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Cloning of 1547 PiggyBacHR-SMN1-3HA
[edit]Purpose: Cloning of 1547 PiggyBacHR-SMN1-3HA
Date: 2013-1-28---2013-1-30
Notebook:0
Page:
Clone ID:1547
Experimenters: Shanyi
Results
[edit]PCR
[edit]PCR from 293 genomic DNA
digest with Sap1 + Mfe1, clone into EcoR1 + Sap1 digested 1507 PiggyBacHR
EcoR1 in the vector is gone, but there are two internal EcoR1 sites
| band 2 |
band 3,4,5 | |
| 1507 |
1547PCR | |
| enzymes |
SapI + Mfe1/EcoR1 | |
Colony Screening
[edit]Date: 2013-
Make miniprep and digest withEcoR1=>215bp
Result intepretation/Summary
[edit]- <br>
Conclusion
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