1560 pT7A3E-T4-Endo-IV
Cloning of 1560 pT7A3E-T4-Endo-IV
[edit]Purpose: Cloning of1560 pT7A3E-T4-Endo-IV==
Date: 2014-2----
Notebook:0
Page:
Clone ID:1560
Experimenters: shanyi
Results
[edit]CLONING
[edit]Gene synthesis
digest with NdeI+XhoI
Colony Screening
[edit]Date: 2014
Make miniprep, digest with NdeI+XhoI
SENT 1560-5 TO SEQUENCE,missing 1 G, can be fixed by PCR using primer
T4-Endo-IV-1 TAGCACCATATGCAGAAAACGAATCCTGGGTTACAGCGTCTGTTTCAGATTCCGACCT
T4-Endo-IV-14 GGTCGTACTCGAGAATGGAAAGATACCATCCGTTGTAGTTGCTTTTGC
Cloning of 1560 pT7A3E-T4-Endo-IV
[edit]Purpose: Cloning of1560 pT7A3E-T4-Endo-IV==
Date: 2014-2----
Notebook:0
Page:
Clone ID:1560
Experimenters: shanyi
Results
[edit]CLONING
[edit]pcr
digest with NdeI+XhoI
Colony Screening
[edit]Date: 2014
Make miniprep, digest with NdeI+XhoI
Result intepretation/Summary
[edit]Conclusion
[edit]Cloning of 1560_pT7A3E-T4-Endo-I
[edit]'Purpose: Cloning of 1560_pT7A3E-T4-Endo-I==
Date: 2014-5----
Notebook:0
Page:
Clone ID:1560
Experimenters: shanyi
Results
[edit]CLONING
[edit]subclone from 2132 to 1892 using Nde1 + Xho1
Colony Screening
[edit]Date: 2014
Make miniprep, digest with Nde1 + Xho1
