1561 1946 1950 1931 1932 1933 2017 2025 2026

Protein Expression

2014/1/24
SHANYI

Lane	Name	Protein Name	Clone ID	Host	Induction	Purification	pI/Mw
1	PageRuler Plus Prestained protein ladder
2	pT7A3-T4-Endo-V	T4-Endo-V	1561	5 ml, Nico21(DE3)plysN	0.2 mM IPTG  RT o/n	Ni beads	9.52 / 18.2	yes
3	pT7A3E-bTdT	terminal deoxynucleotidyl transferase	1946	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	7.67 / 45.7	yes,but cells grow slowly
4	pT7A3E-Fpg	Fpg	1950	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	8.47 / 31.9	yes,but cells grow slowly
5	pT7A3-T4-RNA-Lig2	T4-RNA-Lig2	1931	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	6.28 / 39.1
6	pT7A3E-T4_RNA_Ligase_ 2_Truncated	T4_RNA_Ligase_ 2_Truncated	1932	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	6.16 / 29.7	cell almost dead
7	pT7A3E-T4RNA-Lig2-truncated-KQ	T4RNA-Lig2-truncated-KQ	1933	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	6.21 / 30
8	pT7A3E-T4RNA-Lig2K227Q	T4RNA-Lig2K227Q	2017	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	6.21 / 30
9	pT7A3E-T4-Ligase-HNH-6H	T4-Ligase-HNH-6H	2025	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	6.50 / 61.7
10	pT7A3E-T4-Ligase-HI-6H	T4-Ligase-HI-6H	2026	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	6.39 / 69.0