1571-pT7A3-APE1
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Cloning of 1571-pCMV5-BirA
[edit]Purpose: Cloning of 2216-pCMV5-BirA
Date: 2014-7-01---2014-7-09
Notebook:0
Page:
Clone ID:1571
Experimenters: LIANGHUO
Results
[edit]cloning
[edit]PCR from 1559 pLX304-APE1
APE1-Nco1-F
ctagcaCCATG GGC AGCAGCCATCATCATCATCATCAC CCGAAGCGTGGGAAAAAGGGAG
APE1-Xho1-R
(CTATCACCCTATACCTAGCACTG CTCGAG ctagaa)
ttctagCTCGAGCAGTGCTAGGTATAGGGTGATAG
1571 DIGESTED
[edit]
Colony Screening
[edit]Date: 2014-7-09
Make miniprep and digest with Nde1 + XhoI
SELECT CLONE3 FOR SEQUENCING
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Result intepretation/Summary
[edit]Conclusion
[edit]